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Sperm Freezing

Sperm freezing is a short laboratory procedure in which a semen sample is analysed, processed, and stored at a very low temperature for potential future use. It is an established way to preserve reproductive potential before treatment, surgery, or for a time that may be more appropriate for you.

Duration

Providing and processing a standard semen sample is usually completed within a single visit.

Stay at the Clinic

No stay is required. After providing the sample, you can continue with your usual daily activities.

Feeling

Standard semen sample collection is not associated with pain and does not require an injection.

Storing Reproductive Cells for Future Use

What Exactly Is Frozen?

What Does It Mean?

  • A semen sample contains sperm cells and fluid produced by the male reproductive system.
  • In the laboratory, we assess the sample and prepare suitable sperm cells for storage.
  • The material is divided into separate portions and placed in carefully labelled cryogenic containers.

What Is Important to Know?

  • A cryoprotectant is added to the material to help protect the cells during freezing.
  • The samples are stored in a controlled environment at temperatures below −130°C, usually in liquid nitrogen or its vapour.
  • The procedure preserves reproductive cells but does not guarantee a future pregnancy or live birth.

Medical and Personal Reasons for Cryopreservation

When Can We Discuss Sperm Freezing?

  • 1

    1. Before Treatment or Surgery

    Cryopreservation may be discussed before chemotherapy, radiotherapy, or other treatment that could affect sperm production.
    It may also be appropriate before surgery involving the testes, prostate, or pelvis that could affect spermatogenesis or ejaculation.
  • 2

    2. As Part of Fertility Treatment

    A sample may be frozen in advance for the day of an insemination or IVF procedure.
    This may be helpful if providing a sample is difficult, in cases of frequent travel, working abroad, or variable semen analysis results.
  • 3

    3. When the Sperm Count Is Very Low

    When sperm concentration is low, we may recommend storing material from more than one sample.
    Sperm obtained through procedures involving the epididymis or testis may also be frozen for future ICSI fertilisation.
  • 4

    4. For Personal Reasons

    Freezing may be discussed before vasectomy, certain hormonal treatments, or when your future plans have not yet been finalised.
    The reason for the procedure, your health, and the quality of the sample at the time of freezing are important considerations.
How Should You Prepare?
We begin with a discussion of your medical history, previous surgery, medications, infections, recent high fever, and the reason for freezing.
When necessary, we may recommend semen analysis and additional tests according to the planned future use.
A period of two to seven days of sexual abstinence is usually recommended, but you will receive individual instructions from our team.
What Does the Semen Analysis Show?
Semen analysis assesses sample volume, sperm concentration, and sperm motility.
Depending on the individual case, morphology, vitality, and other parameters may also be assessed.
The results do not determine your ability to become a parent. They help us select an appropriate laboratory and treatment plan.

The Faces of Hope

How Is the Sample Provided?

The sample is usually provided by masturbation into a sterile container, in a private setting intended for this purpose.

It is important that the entire sample is collected and that the container remains correctly labelled.

If providing a sample is difficult or impossible, please let us know in advance. We will discuss possible options according to the medical reason and your comfort.

How Does Cryopreservation Work?

After the semen has liquefied, the embryologist assesses the main parameters of the sample.

A cryoprotective medium is added to the prepared material. It is then divided into portions, placed in cryogenic containers, and frozen according to a controlled laboratory protocol.

At every stage, identification, traceability, and documentation rules are followed.

Laboratory Assessment and Controlled Storage

How Is the Procedure Performed at Adella Clinic?

What Happens in the Laboratory?

  • The semen sample is allowed to liquefy and its main parameters are assessed.
  • When necessary, a test of sperm viability after freezing may also be performed.
  • The material is processed and divided into separate portions.
  • The samples are placed in cryogenic containers and stored at a controlled ultra-low temperature.

What Happens After the Procedure?

  • You will receive information about the quality of the sample and the number of portions stored.
  • We will discuss whether there is a reason to provide an additional sample.
  • After standard semen sample collection, there is no recovery period.
  • If another sample is needed, we will determine the appropriate interval.

Thawing and Options for Future Treatment

How Can the Stored Sperm Be Used?

When you decide to use the stored material, one or more portions are thawed according to the laboratory protocol. We then assess the number of viable and motile sperm cells.

If there is a sufficient number of motile sperm cells, intrauterine insemination may be considered. In other cases, the material may be used for conventional IVF or ICSI.

The choice depends not only on the semen sample, but also on egg quality, ovarian reserve, and the overall treatment plan.

What Happens If No Sperm Are Found?

  • The absence of sperm in a single sample does not by itself determine all future options.
  • A repeat test or consultation with an andrologist may be recommended.
  • When medically indicated, PESA, MESA, TESE, or micro-TESE may be discussed.
  • If viable sperm are found, they may be frozen for future use, most commonly with ICSI.

What Are Realistic Expectations?

  • The outcome depends on the quality of the sample before freezing and the number of portions stored.
  • The characteristics and motility of sperm may change after thawing.
  • The selected treatment method, egg quality, and embryo development are also important.
  • Freezing preserves reproductive cells but does not guarantee a future pregnancy or live birth.

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